Ageritin from poplar mushrooms: scale-up purification and cytotoxicity towards undifferentiated and differentiated SH-SY5Y cells.
Identifieur interne : 000B45 ( Main/Exploration ); précédent : 000B44; suivant : 000B46Ageritin from poplar mushrooms: scale-up purification and cytotoxicity towards undifferentiated and differentiated SH-SY5Y cells.
Auteurs : Sara Ragucci [Italie] ; Severina Pacifico ; Maria Rosaria Ruocco ; Giuseppina Crescente ; Rosarita Nasso ; Martina Simonetti ; Mariorosario Masullo ; Simona Piccolella ; Paolo V. Pedone ; Nicola Landi ; Antimo Di MaroSource :
- Food & function [ 2042-650X ] ; 2019.
Descripteurs français
- KwdFr :
- Agaricales (composition chimique), Apoptose (effets des médicaments et des substances chimiques), Caspase-3 (génétique), Caspase-3 (métabolisme), Différenciation cellulaire (effets des médicaments et des substances chimiques), Extraits de plantes (composition chimique), Extraits de plantes (isolement et purification), Extraits de plantes (pharmacologie), Humains (MeSH), Lignée cellulaire tumorale (MeSH), Neurones (cytologie), Neurones (effets des médicaments et des substances chimiques), Neurones (métabolisme), Ribonucléases (composition chimique), Ribonucléases (isolement et purification), Ribonucléases (pharmacologie).
- MESH :
- composition chimique : Agaricales, Extraits de plantes, Ribonucléases.
- cytologie : Neurones.
- effets des médicaments et des substances chimiques : Apoptose, Différenciation cellulaire, Neurones.
- génétique : Caspase-3.
- isolement et purification : Extraits de plantes, Ribonucléases.
- métabolisme : Caspase-3, Neurones.
- pharmacologie : Extraits de plantes, Ribonucléases.
- Humains, Lignée cellulaire tumorale.
English descriptors
- KwdEn :
- Agaricales (chemistry), Apoptosis (drug effects), Caspase 3 (genetics), Caspase 3 (metabolism), Cell Differentiation (drug effects), Cell Line, Tumor (MeSH), Humans (MeSH), Neurons (cytology), Neurons (drug effects), Neurons (metabolism), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Plant Extracts (pharmacology), Ribonucleases (chemistry), Ribonucleases (isolation & purification), Ribonucleases (pharmacology).
- MESH :
- chemical , chemistry : Plant Extracts, Ribonucleases.
- chemical , genetics : Caspase 3.
- chemistry : Agaricales.
- cytology : Neurons.
- drug effects : Apoptosis, Cell Differentiation, Neurons.
- chemical , isolation & purification : Plant Extracts, Ribonucleases.
- chemical , metabolism : Caspase 3, Neurons.
- chemical , pharmacology : Plant Extracts, Ribonucleases.
- Cell Line, Tumor, Humans.
Abstract
Ageritin is the first reported ribotoxin-like protein from basidiomycetes fungi. It can induce ribosomal integrity damage and translation block, and interferes with mitochondrial redox activity of some glioma and neuroblastoma cell lines. Herein, Ageritin has been investigated as a valuable neurotoxin towards either undifferentiated or retinoic acid (RA)-differentiated SH-SY5Y neuroblastoma cells showing a selective cell toxicity against undifferentiated cells. MTT and sulforhodamine B (SRB) assays highlighted that Ageritin markedly decreases the mitochondrial redox activity and viability of undifferentiated cells, meanwhile inducing evident morphological changes eliciting neuronal-like appearance in these cells. Data from lactate dehydrogenase release assay, cytofluorimetric analysis and caspase-3 enzymatic activity measurement suggest that Ageritin promotes cell death through a caspase-dependent apoptotic pathway. The Z-VAD-FMK caspase inhibitor was able to prevent this apoptotic pathway activation. Based on the interesting behaviour of Ageritin vs. SH-SY5Y cells, the development of a scale-up procedure to obtain the purified protein in larger amounts (yield 2.5 mg per 100 g) has been optimized.
DOI: 10.1039/c9fo01483g
PubMed: 31441483
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Pedone</name></author><author><name sortKey="Landi, Nicola" sort="Landi, Nicola" uniqKey="Landi N" first="Nicola" last="Landi">Nicola Landi</name></author><author><name sortKey="Di Maro, Antimo" sort="Di Maro, Antimo" uniqKey="Di Maro A" first="Antimo" last="Di Maro">Antimo Di Maro</name></author></analytic><series><title level="j">Food & function</title><idno type="eISSN">2042-650X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (chemistry)</term><term>Apoptosis (drug effects)</term><term>Caspase 3 (genetics)</term><term>Caspase 3 (metabolism)</term><term>Cell Differentiation (drug effects)</term><term>Cell Line, Tumor (MeSH)</term><term>Humans (MeSH)</term><term>Neurons (cytology)</term><term>Neurons (drug effects)</term><term>Neurons (metabolism)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (isolation & purification)</term><term>Plant Extracts (pharmacology)</term><term>Ribonucleases (chemistry)</term><term>Ribonucleases (isolation & purification)</term><term>Ribonucleases (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agaricales (composition chimique)</term><term>Apoptose (effets des médicaments et des substances chimiques)</term><term>Caspase-3 (génétique)</term><term>Caspase-3 (métabolisme)</term><term>Différenciation cellulaire (effets des médicaments et des substances chimiques)</term><term>Extraits de plantes (composition chimique)</term><term>Extraits de plantes (isolement et purification)</term><term>Extraits de plantes (pharmacologie)</term><term>Humains (MeSH)</term><term>Lignée cellulaire tumorale (MeSH)</term><term>Neurones (cytologie)</term><term>Neurones (effets des médicaments et des substances chimiques)</term><term>Neurones (métabolisme)</term><term>Ribonucléases (composition chimique)</term><term>Ribonucléases (isolement et purification)</term><term>Ribonucléases (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Extracts</term><term>Ribonucleases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Caspase 3</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Agaricales</term><term>Extraits de plantes</term><term>Ribonucléases</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Neurones</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term><term>Cell Differentiation</term><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Apoptose</term><term>Différenciation cellulaire</term><term>Neurones</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Caspase-3</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Plant Extracts</term><term>Ribonucleases</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Extraits de plantes</term><term>Ribonucléases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Caspase 3</term><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Caspase-3</term><term>Neurones</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Extraits de plantes</term><term>Ribonucléases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Plant Extracts</term><term>Ribonucleases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Lignée cellulaire tumorale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ageritin is the first reported ribotoxin-like protein from basidiomycetes fungi. It can induce ribosomal integrity damage and translation block, and interferes with mitochondrial redox activity of some glioma and neuroblastoma cell lines. Herein, Ageritin has been investigated as a valuable neurotoxin towards either undifferentiated or retinoic acid (RA)-differentiated SH-SY5Y neuroblastoma cells showing a selective cell toxicity against undifferentiated cells. MTT and sulforhodamine B (SRB) assays highlighted that Ageritin markedly decreases the mitochondrial redox activity and viability of undifferentiated cells, meanwhile inducing evident morphological changes eliciting neuronal-like appearance in these cells. Data from lactate dehydrogenase release assay, cytofluorimetric analysis and caspase-3 enzymatic activity measurement suggest that Ageritin promotes cell death through a caspase-dependent apoptotic pathway. The Z-VAD-FMK caspase inhibitor was able to prevent this apoptotic pathway activation. Based on the interesting behaviour of Ageritin vs. SH-SY5Y cells, the development of a scale-up procedure to obtain the purified protein in larger amounts (yield 2.5 mg per 100 g) has been optimized.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31441483</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2042-650X</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>10</Issue><PubDate><Year>2019</Year><Month>Oct</Month><Day>16</Day></PubDate></JournalIssue><Title>Food & function</Title><ISOAbbreviation>Food Funct</ISOAbbreviation></Journal><ArticleTitle>Ageritin from poplar mushrooms: scale-up purification and cytotoxicity towards undifferentiated and differentiated SH-SY5Y cells.</ArticleTitle><Pagination><MedlinePgn>6342-6350</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c9fo01483g</ELocationID><Abstract><AbstractText>Ageritin is the first reported ribotoxin-like protein from basidiomycetes fungi. It can induce ribosomal integrity damage and translation block, and interferes with mitochondrial redox activity of some glioma and neuroblastoma cell lines. Herein, Ageritin has been investigated as a valuable neurotoxin towards either undifferentiated or retinoic acid (RA)-differentiated SH-SY5Y neuroblastoma cells showing a selective cell toxicity against undifferentiated cells. MTT and sulforhodamine B (SRB) assays highlighted that Ageritin markedly decreases the mitochondrial redox activity and viability of undifferentiated cells, meanwhile inducing evident morphological changes eliciting neuronal-like appearance in these cells. Data from lactate dehydrogenase release assay, cytofluorimetric analysis and caspase-3 enzymatic activity measurement suggest that Ageritin promotes cell death through a caspase-dependent apoptotic pathway. The Z-VAD-FMK caspase inhibitor was able to prevent this apoptotic pathway activation. Based on the interesting behaviour of Ageritin vs. SH-SY5Y cells, the development of a scale-up procedure to obtain the purified protein in larger amounts (yield 2.5 mg per 100 g) has been optimized.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ragucci</LastName><ForeName>Sara</ForeName><Initials>S</Initials><Suffix></Suffix><AffiliationInfo><Affiliation>Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania 'Luigi Vanvitelli', Via Vivaldi 43, 81100-Caserta, Italy. antimo.dimaro@unicampania.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pacifico</LastName><ForeName>Severina</ForeName><Initials>S</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Ruocco</LastName><ForeName>Maria Rosaria</ForeName><Initials>MR</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Crescente</LastName><ForeName>Giuseppina</ForeName><Initials>G</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Nasso</LastName><ForeName>Rosarita</ForeName><Initials>R</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Simonetti</LastName><ForeName>Martina</ForeName><Initials>M</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Masullo</LastName><ForeName>Mariorosario</ForeName><Initials>M</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Piccolella</LastName><ForeName>Simona</ForeName><Initials>S</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Pedone</LastName><ForeName>Paolo V</ForeName><Initials>PV</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Landi</LastName><ForeName>Nicola</ForeName><Initials>N</Initials><Suffix></Suffix></Author><Author ValidYN="Y"><LastName>Di Maro</LastName><ForeName>Antimo</ForeName><Initials>A</Initials><Suffix></Suffix></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Food Funct</MedlineTA><NlmUniqueID>101549033</NlmUniqueID><ISSNLinking>2042-6496</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.-</RegistryNumber><NameOfSubstance UI="D012260">Ribonucleases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.-</RegistryNumber><NameOfSubstance UI="C000632196">ageritin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="C505407">CASP3 protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D053148">Caspase 3</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000363" MajorTopicYN="N">Agaricales</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053148" MajorTopicYN="N">Caspase 3</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009474" MajorTopicYN="N">Neurons</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012260" MajorTopicYN="N">Ribonucleases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31441483</ArticleId><ArticleId IdType="doi">10.1039/c9fo01483g</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Crescente, Giuseppina" sort="Crescente, Giuseppina" uniqKey="Crescente G" first="Giuseppina" last="Crescente">Giuseppina Crescente</name><name sortKey="Di Maro, Antimo" sort="Di Maro, Antimo" uniqKey="Di Maro A" first="Antimo" last="Di Maro">Antimo Di Maro</name><name sortKey="Landi, Nicola" sort="Landi, Nicola" uniqKey="Landi N" first="Nicola" last="Landi">Nicola Landi</name><name sortKey="Masullo, Mariorosario" sort="Masullo, Mariorosario" uniqKey="Masullo M" first="Mariorosario" last="Masullo">Mariorosario Masullo</name><name sortKey="Nasso, Rosarita" sort="Nasso, Rosarita" uniqKey="Nasso R" first="Rosarita" last="Nasso">Rosarita Nasso</name><name sortKey="Pacifico, Severina" sort="Pacifico, Severina" uniqKey="Pacifico S" first="Severina" last="Pacifico">Severina Pacifico</name><name sortKey="Pedone, Paolo V" sort="Pedone, Paolo V" uniqKey="Pedone P" first="Paolo V" last="Pedone">Paolo V. Pedone</name><name sortKey="Piccolella, Simona" sort="Piccolella, Simona" uniqKey="Piccolella S" first="Simona" last="Piccolella">Simona Piccolella</name><name sortKey="Ruocco, Maria Rosaria" sort="Ruocco, Maria Rosaria" uniqKey="Ruocco M" first="Maria Rosaria" last="Ruocco">Maria Rosaria Ruocco</name><name sortKey="Simonetti, Martina" sort="Simonetti, Martina" uniqKey="Simonetti M" first="Martina" last="Simonetti">Martina Simonetti</name></noCountry><country name="Italie"><noRegion><name sortKey="Ragucci, Sara" sort="Ragucci, Sara" uniqKey="Ragucci S" first="Sara" last="Ragucci">Sara Ragucci</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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